Effects of calcitriol on the expression of vitamin D receptor. RANKL and osteoprotegerin in human periodontal ligament cells
10.3321/j.issn:1002-0098.2008.12.008
- VernacularTitle:骨化三醇对人牙周膜细胞维生素D受体、RANKL和骨保护因子表达的调节
- Author:
Xiao-Lin TANG
1
;
Huan-Xin MENG
;
Li ZHANG
Author Information
1. 中国医科大学口腔医学院
- Keywords:
Calcitriol;
Receptor activator of nuclear factor-kappa B;
Osteoprotegerin;
Human periodontal ligament cells
- From:
Chinese Journal of Stomatology
2008;43(12):732-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of 1,25-dihydroxyvitamin D3(VD3)on the expression of vitamin D receptor(VDR),receptor activator of nuclear factor-kappa B ligand(RANKL)and osteoprotegerin(OPG)in human periodontal ligament cells(hPDLC)populations and to analyze the potential mechanisms.Methods Twelve hPDLC populations were primarily established from 12 donors individually.Two samples of each hPDLC population of passage three were treated respectively with 10-8 mol/L VD3(V D3 group)or 0.1% absolute ethyl alcohol as controls(control group).Six days later,the mRNA expression Ievels of VDR.RANKL and OPG in the samples were determined with real-time quantitative RT-PCR.The DNA base sequences upstream to the transcription start site of RANKL gene were also analyzed.Results Compared with the control group.the mRNA expression level of VDR increased significantly in the VD3 group(P=0.003),averagely(3.04±1.06)times of that in the control group;the mRNA expression level of RANKL was also up-regulated by VD3(P=0.001),9.82(0.75-119.18)times of that in the control group;the OPG expression level was(94.48±39.15)% of the controls(P=0.136);OPG/RANKL ratio was down-regulated in the VD3 group to averagely 10.36%(1.01%-138.00%)of the controls(P=0.003).No mutation wes found in the DNA fragments upstream to the transcription start site in the RANKL gene and the genotypes of the polymorphism at-1832(rs7984870,C/G)were not shown to be significantly related to the RANKL mRNA expression leveL Conclusions In hPDLC,VD3 can significantly increase the mRNA expression level of VDR:VD3 can increase RANKL mRNA expression level to decrease OPG/RANKL ratio,but it has little effect on OPG mRNA expression.The big differences of the RANKL mRNA regulation in response to VD3 treatment among hPDLC populations may not be associated with the DNA sequences upstream to the transcription start site in the RANKL gene.
