Roles of matrix metalloproteinases and tissue specific inhibitors of metalloproteinases in dentinogenic ghost cell tumor and ghost cell odontogenic carcinoma.
- Author:
Ning GENG
1
;
Yu BAN
;
Yu CHEN
;
Ming-Zhong YANG
;
Dong-Mei BAO
;
Xin-Zhu YI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Dentin; Humans; Mandibular Neoplasms; metabolism; pathology; Matrix Metalloproteinases; metabolism; Middle Aged; Odontogenic Tumors; metabolism; pathology; Tissue Inhibitor of Metalloproteinases; metabolism; Young Adult
- From: Chinese Journal of Stomatology 2008;43(12):756-760
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the roles of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in dentinogenic ghost cell tumor (DGCT) and ghost cell odontogenic carcinoma (GCOC).
METHODSThe expressions of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry. Their mRNA expression in one DGCT case and one GCOC case were investigated by RT-PCT.MMP-2 and MMP-9 protein activities in the two cases were analyzed by gelatin zymography.
RESULTSMMP-9 and TIMP-1 expressions elevated greatly in GCOC, and there was a significant difference (P < 0.05) in TIMP-1 expression between GCOC and DGCT.Pro-MMP-9, MMP-9 activated form, pro-MMP-2, and MMP-2 activated forms were detected in the GCOC case, while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case. The mRNA level of MMP-9 elevated obviously in the GCOC case, which was similar to that of TIMP-1.
CONCLUSIONSThe elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.
