Roles of matrix metalloproteinases and tissue specific inhibitors of metalloproteinases in dentinogenic ghost cell tumor and ghost cell odontogenic carcinoma

VernacularTitle:基质金属蛋白酶及其组织抑制剂在牙本质生成性影细胞瘤和牙源性影细胞癌中的表达
Author: Ning GENG ¹ ; Yu BAN ; Yu CHEN ; Ming-Zhong YANG ; Dong-Mei BAO ; Xin-Zhu YI
Author Information

1. 四川大学
Keywords: Matrix metalloproteinases; Tissue inhibitor of metallopmteinases; Odontogenic tumor; Ghost cell
From: Chinese Journal of Stomatology 2008;43(12):756-760
CountryChina
Language:Chinese
Abstract: Objective To investigate the roles of matrix metalloproteinases(MMP)and tissue inhibitors of metalloproteinases(TIMP)in dentinogenic ghost cell tumor(DGCT)and ghost cell odontogenic carcinoma(GCOC).Methods The expressions of MMP-2,MMP-9,MMP-14,TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry.Their mRNA expression in one DGCT case and one GCOC case were investigated by RT-PCT.MMP-2 and MMP-9 protein activities in the two csses were analyzed by gelatin zymography.Results MMP-9 and TIMP-1 expressions elevated greatly in GCOC,and there was a significant dliferenee(P＜0.05)in TIMP-1 expression between GCOC and DGCT.Pro-MMP-9,MMP-9 activated form,pro-MMP-2,and MMP-2 activated forms were detected in the GCOC case.while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case.The mRNA level of MMP-9 elevated obviously in the GCOC case,which was similar to that of TIMP-1.Conclusions The elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.