Effect of chemokine receptor 7 small interfering RNA on proliferation and invasion of squamous cell carcinoma of head and neck
10.3760/cma.j.issn.1002-0098.2009.01.003
- VernacularTitle:趋化因子受体7小干扰RNA对人头颈部鳞状细胞癌细胞增殖及侵袭的影响
- Author:
Zhen-Jin ZHAO
1
;
Fa-Yu LIU
;
Chong-Fu SUN
Author Information
1. 中国医科大学口腔医学院
- Keywords:
Carcinoma,squamous cell;
Receptors,chemokine;
Neoplasm invasiveness
- From:
Chinese Journal of Stomatology
2009;44(1):5-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of chemokine receptor 7(CCR-7)small interfering RNA (siRNA)on proliferation and invagion of squamous cell carcinoma of head and neck(SCCHN).Methods CCR-7 siRNA was co-transfected into SCCHN cell line PCI-4B using LipofectamineTM 2000.CCR-7 protein level was detected by western blotting.SCCHN cell proliferation was detected by MTT,and the change of actin cytoskeleton observed by confocal laser scanning microscope.Transwell assays were used to determine chemotaxis and invasion of SCCHN cells.The activity and nuclear translocation of nuclear factor-kappa B (NF-κB)were detected by TransAMTM NF-κB p65 kit and fluorescence microscope respectively.Results After CCR-7 siRNA transfection.the protein level of CCR-7 was significantly decreased.The changes induced by CCL-19,including increased proliferation rate,polarized actin polymerization,increased chemotaxis rate and invasion rate,were all abolished by CCR-7 siRNA transfection.CCR-7 siRNA also diminished CCL-19-induced NF-κB activation and nuclear translocation.Conclusions CCR-7 siRNA could inhibit expression of CCR-7 and diminish the increased proliferation and invasion of SCCHN induced by CCL-19 in vitro.CCR-7 siRNA may provide a potential treatment strategy for SCCHN.
