Effect of Porphyromonas gingivalis on nitric oxide in cultured human umbilical vein endothelial cells

VernacularTitle:牙龈卟啉单胞菌对人脐静脉内皮细胞一氧化氮生成的影响
Author: Juan WU ¹ ; Wei-Bin SUN ; Yong JI
Author Information

1. 南京医科大学附属口腔医院
Keywords: Porphyromonas gingivalis; Umbilical veins; Endothelial cells; Nitric oxide
From: Chinese Journal of Stomatology 2009;44(1):24-27
CountryChina
Language:Chinese
Abstract: Objective To observe the effect of Porphyromonas gingivalis(Pg)on the production of nitric oxide(NO)in cultured human umbilical vein endothelial cells(HUVEC).and to investigate the pathway of damaging endothelial function by Pg.Methods Pg ATCC33277 was cultured in anaerobic jar,and HUVEC was treated with various concentrations of Pg ATCC33277 at multiplicity of infection(MOI)of 1:10,1:100 and 1:1000 for 4,8,12,24 h respectively.The cells supernatants were collected and stored at-70℃and NO concentration in the cells supematanta was measured by nitrate reductase assay.Results Within 24 h,Pg at MOI of 1:10 and 1:100 stimulated the release of nitric oxide in cultured HUVEC.Within 12 h,Pg at an MOI of 1:1000 group increased NO production,and NO decreased at 24 h.Conclusions Pg has an effect on the production of NO.Low concentrations of Pg stimulated release of nitric oxide in endothelial cells but high concentrations can decrease the release of NO.