In vitro analysis of intake of minocycline by mature rat mandibular osteoblasts.
- Author:
Wen-Yi ZHANG
1
;
Hong-Chen LIU
;
Xia WU
;
Ling-Ling E
;
Yan LÜ
;
Jian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Mandible; cytology; drug effects; metabolism; Minocycline; pharmacokinetics; Osteoblasts; drug effects; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Stomatology 2009;44(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the intake of minocycline and its amount in mature rat mandibular osteoblasts (MRMOB) in vitro, and to identify the feasibility of intracellular anti-bacterial activity of minocycline.
METHODSFour groups of MRMOB were incubated in 100 mg/L minocycline for 15, 30, 45 and 60 minutes respectively, and the accumulation of minocycline within MRMOB was measured using a fluorescence spectrophotometer.
RESULTSThe intracellular accumulation amount of minocycline in the four groups of MRMOB was (17.29 +/- 1.49), (16.87 +/- 1.57), (16.96 +/- 1.67) and (17.94 +/- 1.63) mg/g respectively after osteoblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained minocycline for 15, 30, 45 and 60 min. There was no significant difference in amount of minocycline among the four groups of MRMOB.
CONCLUSIONSThe mature rat mandibular osteoblasts can ingest minocycline, and the accumulation amount of minocycline in MRMOB is irrelevant with the exposure time of MRMOB to minocycline.
