In vitro analysis of intake of minocycline by mature rat mandibular osteoblasts

VernacularTitle:鼠下颌骨成骨细胞对米诺环素摄取的实验观察
Author: Wen-Yi ZHANG ¹ ; Hong-Chen LIU ; Xia WU ; Ling-Ling E ; Yan L(U) ; Jian ZHANG
Author Information

1. 天津医科大学口腔医院
Keywords: Osteoblast; Minocycline; Animal experimentation
From: Chinese Journal of Stomatology 2009;44(1):28-31
CountryChina
Language:Chinese
Abstract: Objective To observe the intake of minocycline and its amount in mature rat mandibular oateoblasts(MRMOB)in vitro,and to identify the feasibility of intracellular anti-bacterial activity of minocycline.Methods Four groups of MRMOB were incubated in 100 mg/L minocycline for 15,30,45 and 60 minutes respectively,and the accumulation of minocycline within MRMOB was measured using a fluorescence spectrophotometer.Results The intracellular accumulation amount of minocycline in the four groups of MRMOB was(17.29±1.49),(16.87±1.57),(16.96±1.67)and(17.94±1.63)mg/g respectively after osteoblasts were cultured in Dulbecco's modified Eagle's medium(DMEM)which contained minocycline for 15,30,45 and 60 min.There was no significant difference in amount of minocycline among the four groups of MRMOB.Conclusions The mature rat mandibular ostoeblasts can ingest minocycline.and the accumulation amount of minocycline in MRMOB is irrelevant with the exposure time of MRMOB to minocycline.