Detection of luxS gene of quorum sensing signal system in periodontal pathogen.
- Author:
Chao-Feng LEI
1
;
He YANG
;
Chang-Juan SUN
;
Di MIAO
;
Yi XU
Author Information
- Publication Type:Journal Article
- MeSH: Aggregatibacter actinomycetemcomitans; genetics; metabolism; Bacterial Proteins; genetics; isolation & purification; metabolism; Carbon-Sulfur Lyases; genetics; isolation & purification; metabolism; Fusobacterium nucleatum; genetics; metabolism; Gene Expression Regulation, Bacterial; Porphyromonas gingivalis; genetics; metabolism; Quorum Sensing; genetics; Signal Transduction
- From: Chinese Journal of Stomatology 2009;44(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the presence and distribution of luxS gene in quorum sensing signal system in the periodontal pathogens.
METHODSThe total DNA of Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Actinobacillus acitinomycetimcomtans (Aa) were extracted. The presence of luxS was detected by polymerase chain reaction (PCR). The products of PCR were detected by electrophoresis, sequenced and identified by a Blast search of the GenBank database.
RESULTSElectrophoresis, sequencing and Blast searching indicated that the PCR products of Pg were highly consistent with the luxS gene in GenBank. The sequencing result of Fn was also identified with the target gene. The PCR product of Aa was the same as reference through electrophoresis.
CONCLUSIONSPg, Fn, Aa contain luxS gene. Further studies may be required to investigate the functions of luxS in the periodontal pathogens.
