The mechanism of inhibitory effect on parotid gland secretion with local injection of botulinum toxin type A in the rat.
- Author:
Wei-Dong WEN
1
;
Fang YUAN
;
Yi-Ping HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Botulinum Toxins, Type A; pharmacology; Female; Parotid Gland; drug effects; secretion; Rats; Rats, Wistar; Sialorrhea; drug therapy; Vasoactive Intestinal Peptide; secretion
- From: Chinese Journal of Stomatology 2009;44(1):38-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the mechanism of inhibitory effect of botulinum toxin type A (BTX-A) on parotid gland secretion.
METHODSFemale Wistar rats (n = 18) were randomly divided into saline injection group (n = 6) and BTX-A injection group ( n = 12), respectively. 0.1 ml of saline was injected into left parotid gland and 2.5 U of BTX-A injected into right parotid gland. Rats were sacrificed at day 7, 12 and 35 post-injections respectively for morphology and vasoactive intestinal polypeptide (VIP) immunoreactivity of parotid gland.
RESULTSFollowing BTX-A injection, some atrophic cells and reduction of number of VIP-immunoreactive (VIP-IR) fibers were found in gland and tube at day 7 (P < 0.05); at day 12, there was more obvious reduction of VIP-IR fibers around tube and vessels and atrophy of cells in BTX-A injection gland than saline injection gland (P < 0.001); at day 35, the glandular cells and VIP-IR fibers were similar to saline injection group.
CONCLUSIONSBTX-A is effective for temporary elimination of hyperfunctioning sialorrhea via inhibition of VIP release which plays a key role in modulation of parotid glands secretion.
