The anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanism.
- Author:
Bing-Xiu XIAO
1
;
Junming GUO
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; pharmacology; Cell Movement; drug effects; Cell Proliferation; drug effects; Genes, myc; Humans; KB Cells; Protein Kinase C; metabolism
- From: Chinese Journal of Stomatology 2009;44(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanisms.
METHODSKB cells were treated with 2.5, 5, 10, 20 and 40 micromol/L aloe-emodin. Crystal violet assay was used to determine the long-term growth inhibition of aloe-emodin on human oral cancer KB cells. Scratch wound-healing motility assay was used to measure the antimigration effect The protein kinase C alpha and c-myc expression changes in protein levels were detected by Western blotting.
RESULTSA durable cell growth inhibitory effect of aloe-emodin on KB cells was found. Treatment of aloe-emodin resulted in the inhibition of cell migration. The protein kinase C alpha and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.
CONCLUSIONSThe anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.
