The anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanism
10.3760/cma.j.issn.1002-0098.2009.01.014
- VernacularTitle:芦荟大黄素抑制人口腔癌KB细胞增殖和迁移的双重效应及其机制研究
- Author:
Bing-Xiu XIAO
1
;
Jun-Ming GUO
Author Information
1. 宁波大学医学院
- Keywords:
Cell growth processes;
Cell movement;
Protein kinase C;
Aloe-emodin
- From:
Chinese Journal of Stomatology
2009;44(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-proliferation and anti-migration dual effects of aloeemodin on KB cells and its mechanisms.Methods KB cells were treated with 2.5,5,10,20 and 40 μmol/L aloe-emodin.Crystal violet assay was used to determine the long-term growth inhibition of aloeemodin on human oral cancer KB cells.Scratch wound-healing motility assay was used to measure the antimigration effect.The protein kinase Cα and c-myc expression changes in protein levels were detected by Western blotting.Results A durable cell growth inhibitory effect of aloe-emodin on KB cells was found.Treatment of aloe-emodin resulted in the inhibition of cell migration.The protein kinase Cα and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.Condusions The anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.
