Relationship of concentration of lactoferrin and lysozyme in saliva and dental caries in primary dentition.
- Author:
Gao-feng HAO
1
;
Huan-cai LIN
Author Information
- Publication Type:Journal Article
- MeSH: Case-Control Studies; Child, Preschool; Dental Caries; epidemiology; metabolism; Female; Humans; Lactoferrin; metabolism; Male; Muramidase; metabolism; Prevalence; Saliva; chemistry; Salivary Proteins and Peptides; Tooth, Deciduous
- From: Chinese Journal of Stomatology 2009;44(2):82-84
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between the concentrations of lactoferrin and lysozyme in saliva and dental caries in primary dentition among Chinese children.
METHODSForty children with high dmft score (dmft > or = 5) and 40 caries-free children (dmft = 0) were sampled and assigned into two groups. Total salivary proteins was measured by means of bicinchoninic acid. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed to analyze the images of target straps. Lactoferrin and lysozyme were detected using Western blotting method.
RESULTSThe total salivary protein in high dmft group [(852.02 +/- 206.14) mg/L] was lower than that of caries-free group [(1032.44 +/- 221.99) mg/L, P < 0.001]. The ratio of 77,000 protein in high dmft group [(12.50 +/- 7.73) IA/microg] was significantly higher than that of the caries-free children [(8.71 +/- 4.28) IA/microg, P = 0.009], while there was no significant difference for 14,500 protein between them (P = 0.137). The ratio of lactoferrin was higher in high dmft group [(229.04 +/- 197.14) IA/microg] than that in caries-free children [(144.07 +/- 99.91) IA/microg, P = 0.018], while no significant difference for lysozyme between the two groups (P = 0.091).
CONCLUSIONSSaliva protein is closely related to caries in primary dentition. Lactoferrin may be one of the important components.