Relationship of concentration of Iactoferrin and lysozyme in saliva and dental caries in primary dentition
10.3760/cma.j.issn.1002-0098.2009.02.006
- VernacularTitle:唾液乳铁蛋白和溶菌酶含量与乳牙患龋的关系
- Author:
Gao-Feng HAO
1
;
Huan-Cai LIN
Author Information
1. 广东省深圳市妇幼保健院
- Keywords:
Tooth,deciduous;
Dental caries;
Lactoferrin;
Muramidase
- From:
Chinese Journal of Stomatology
2009;44(2):82-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the concentrations of lactoferrin and lysozyme in saliva and dental caries in primary dentition among Chinese children. Methods Forty children with high dmft score ( dmft ≥ 5) and 40 caries-free children ( dmft = 0) were sampled and assigned into two groups. Total salivary proteins was measured by means of bicinchoninic acid. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed to analyze the images of target straps. Lactoferrin and lysozyme were detected using Western blotting method. Results The total salivary protein in high dmft group [ ( 852.02 ± 206. 14 ) mg/L ] was lower than that of caries-free group [ ( 1032. 44 ±221.99) mg/L, P < 0. 001 ]. The ratio of 77 000 protein in high draft group[ ( 12. 50 ± 7. 73 ) IA/μg] was significantly higher than that of the caries-free children [ (8.71 ± 4. 28 )IA/μg,P = 0. 009], while there was no significant difference for 14 500 protein between them (P = 0. 137). The ratio of lactoferrin was higher in high draft group[ (229.04 ± 197.14)IA/μg] than that in caries-free children [ (144. 07 ±99.91 ) IA/μg, P = 0. 018 ], while no significant difference for lysozyme between the two groups ( P =0. 091 ). Conclusions Saliva protein is closely related to caries in primary dentition. Lactoferrin may be one of the important components.
