Biologic effects of advanced oxidative protein products on the human gingival fibroblasts
10.3760/cma.j.issn.1002-0098.2009.05.005
- VernacularTitle:晚期蛋白氧化产物对人牙龈成纤维细胞增殖、凋亡及合成基质金属蛋白酶1的影响
- Author:
Yu-Quan DENG
1
;
Yun FU
;
Xiao-Peng SU
;
Zhi-Ying TANG
Author Information
1. 中山大学光华口腔医学院
- Keywords:
Cell proliferation;
Matrix metallopmteinase-1: Apoptosis;
Advanced oxidative protein products
- From:
Chinese Journal of Stomatology
2009;44(5):270-273
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of advanced oxidative protein products(AOPP)on the proliferation,apoptosis and matrix matelloproteinase-1(MMP-1)synthesis of the human gingival flbroblast(HGF).To explore the possible mechanism of the periodontal destruction acceleration in diabetes through AOPP-mediated oxidative stress.Methotis HGF were isolated bv both tissue explant cultivation technique and enzyme digestion method.The culture media with 5、50、100 mg/L AOPP-HAS were added into each experimental group,but the culture media in the control group didn't contain AOPP-HAS.MTT colorimetric assay and ELISA were used to measure the changes of HGF proliferation and the levels of MMP-1 protein from HGF at different time periods.respectively.Seventy-two hours after co-culture with 50 mg/L AOPP-HSA,cell apoptosis was detected by flow cytometry with Annexin V/PI staining.Results Compared to the control group,the growth inhibition rate of HGF in 5、50、100 mg/L AOPP-HSA group was significantly different(P＜0.05).The peak value appeared at 48 hours of co-culture[(19.01±6.28)%,(30.48±5.75)%,(39.75±4.60)%.respectively].There was a dose-dependent relationship between the growth inhibition rate and AOPP-HSA.No significant difference was detected on the apoptotic level between experimental group and the control(P＞0.05).The MMP-1 synthesis in 0.5、5、50、100 mg/LAOPP-HAS group[(55.61±1.06),(65.78±4.04),(79.24±3.09),(89.76±28.88)mg/L,respectively]was significantly higher than that in the control[(34.90±3.15)mg/L]after 72 hours coculture(P＜0.05).There was a dose-dependent relationship between MMP-1 and AOPP-HSA.Conclusions AOPP may inhibit the proliferation of HGF and such effect was not achieved through apeptosis.AOPP may increase collagen degradation by promoting MMP-1 synthesis and thus may accelerate periodontal destruction process in diabetes.
