Effects of gingiival fibroblasts transfected with human transform growth factor-β1 gene on improving the periodontal tissue regeneration
10.3760/cma.j.issn.1002-0098.2009.05.006
- VernacularTitle:人转化生长因子β1基因转染牙龈成纤维细胞促进牙周组织再生的动物实验
- Author:
Qing CHU
1
;
Zhi-Fen WU
;
Guang-Yuan XIE
;
Ling WAN
;
Hai-Li HE
;
Ling-Xia LIU
Author Information
1. 第四军医大学口腔医学院
- Keywords:
Transforming growth factor betal;
Transfection;
Tissue engineering;
Nanostxucmres;
Gingival fibroblast
- From:
Chinese Journal of Stomatology
2009;44(5):274-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of gingival fibroblasts(GF)transfected with hTGF-β1 gene on improving the periodontal tissue regeneration for the repair of degree Ⅱ artificial furcation defects.Methotis The gingival fibroblasts transfected with hTGF-β1 gene was compounded to the cuttlebonetransformed nanometer hydroxyapatite(CBHA)material from the cuttlefish in vitro,the degree Ⅱ furcation defects on the premolars of dogs were produced surgically,and the compound was to implanted into the defect (transfected group),and compared with the compound of periodontal ligament cells(PDLC)with nanometer HA material and the compound of untransfected GF with HA.The results were examined histologically 8 weeks after operation.Results In the transfected group and the positive centrel group,more new attachment was found compared with the negative centrel(P＜0.01),and the NC,NB and NC of the transfected group and the positive control group were: (2.97±0.50),(4.29±0.26)and(4.73±0.06)mm;(3.09±0.26),(4.46±0.25)and(4.69±0.10)mm,respectively.There was no significant diffeFence between the two groups(P＞0.05).Although the alveolar bone regeneration was found in the untransfected group[NB=(3.46±0.32)mm],the root resorption was observed.The tracing experiment showed that the transfected GF were found in the new alveolar bene and the periodontal membrane.Conclusions GF transfected with hTGF-β1 gene can significantly improve the periodontal tissue regeneration in treatment of degree Ⅱ furcation defects and is involved in the formation of the new alveolar bone and the new periodontal membrane.
