Expression and purification of human amelogenin mature peptide in Escherichia coli.
- Author:
Xue-Yang ZHANG
1
;
Jin-Cai ZHANG
;
Hua ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Amelogenin; biosynthesis; genetics; isolation & purification; Electrophoresis, Polyacrylamide Gel; Escherichia coli; metabolism; Glutathione Transferase; genetics; isolation & purification; Humans; Peptides; genetics; isolation & purification; metabolism; Recombinant Fusion Proteins; biosynthesis; genetics; isolation & purification; Recombinant Proteins; genetics; isolation & purification; metabolism
- From: Chinese Journal of Stomatology 2009;44(5):279-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and obtain the purified amelogenin (AMG) mature peptide.
METHODSRecombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21. After expression, AMG was purified with glutathione S-transferase fusion protein purification system (GSTrapFF) column.
RESULTSSodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization results showed that 45,000 GST-AMG fusing protein and 19,000 target AMG mature peptide were obtained successfully.
CONCLUSIONSpGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.
