Expression and purification of human amelogenin mature peptide in Escherichia coil
10.3760/cma.j.issn.1002-0098.2009.05.007
- VernacularTitle:人釉原蛋白成熟肽基因的原核表达和纯化
- Author:
Xue-Yang ZHANG
1
;
Jin-Cai ZHANG
;
Hua ZHAO
Author Information
1. 南方医科大学附属口腔医院(广东省口腔医院)
- Keywords:
Human amelogenin;
Expression;
Purification
- From:
Chinese Journal of Stomatology
2009;44(5):279-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and abtain the purified amelogenin(AMG)mature peptide.Methods Recombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21.After expression,AMG was purified with glutathione S-transferase fusion protein purification system(GSTrapFF)column.Results Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting hybridization results showed that 45 000 GST-AMG fusing protein and 19 000 target AMG mature peptide were obtatined successfully.Conclusions pGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.
