Correlation between the expression of cytokeratin-18-mRNA and invasion and metastasis of oral squamous cell carcinoma.
- Author:
Ping SHU
1
;
De-An ZHAO
;
Da-Peng LU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Squamous Cell; metabolism; pathology; Case-Control Studies; Female; Humans; Keratin-18; genetics; metabolism; Male; Middle Aged; Mouth Neoplasms; metabolism; pathology; Neoplasm Metastasis; RNA, Messenger; genetics
- From: Chinese Journal of Stomatology 2009;44(5):286-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the expression of cytokeratin (CK)-18 and biological behavior of oral squamous cell carcinoma (OSCC).
METHODSTwenty-three patients with OSCC were investigated for the expression of CK-18-mRNA by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Correlations between clinical stages, pathological differentiation, lymphatic metastasis and the expression of CK-18-mRNA were evaluated. CK-18-mRNA expression of peripheral blood from the 23 patients and 23 healthy people were also examined. During follow-up after operation, the peripheral blood was collected again for the expression of CK-19-mRNA.
RESULTSExpression of CK-18-mRNA was found in 16 patients. The expression of CK-18-mRNA was significantly associated with clinical stages, tumor differentiation and lymphatic metastasis. CK-18-mRNA was positive in 4 of 23 blood specimens before operation, but during follow-up only 1 of 23 patients was still positive in peripheral blood.
CONCLUSIONSCK-18 may provide additional information in forecasting the metastasis of OSCC and serve as a reference in monitoring recurrence.
