The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of dentinal tubule.
- Author:
Zhe-Jun WANG
1
;
Yue SA
;
Xiao MA
;
Yi-Ning WANG
;
Tao JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Caseins; Dental Materials; chemical synthesis; Dentin; Dentin Sensitivity; therapy; Durapatite; chemical synthesis; Humans; Materials Testing; Nanostructures; Technology, Dental
- From: Chinese Journal of Stomatology 2009;44(5):297-300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo synthesize nano-hydroxyapatite (nano-HA) and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application.
METHODS(NH(4))(2)HPO(4) and Ca(NO3)2 were used to form nano-HA, which was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FTIR) and X-ray diffractometer (XRD) respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups, which were control group, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM.
RESULTSSEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.
CONCLUSIONSIn comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.
