The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of denfinal tubules
10.3760/cma.j.issn.1002-0098.2009.05.012
- VernacularTitle:纳米羟基磷灰石的制备及其对牙本质小管封闭效果的初步观察
- Author:
Zhe-Jun WANG
1
;
Yue SA
;
Xiao MA
;
Yi-Ning WANG
;
Tao JIANG
Author Information
1. 武汉大学口腔医学院
- Keywords:
Hydroxyapatites;
Dentin sensitivity;
Casein phosphopeptide-amorphous calcium phosphate
- From:
Chinese Journal of Stomatology
2009;44(5):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize nano-hydroxyapatite(nano-HA)and evaluate its effect on the occlusion of dentinal tubules in vitro,and therefore provide evidence for the clinical application.Methods (NH4)2 HPO4 and Ca(NO3)2 were used to form nano-HA,which was characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared spectrometer (FTIR)and X-ray diffractometer(XRD)respectively.Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups,which were control group,casein phosphopetide-amorphous calcium phosphate(CPP-ACP)group,and nano-HA group.After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days,the surface of slab dentin Was observed using SEM.Results SEM,TEM,FTIR,and XRD tests showed that nano-HA was synthesized successfully.SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.Conclusions In comparison with CPP-ACP.nano-HA could occlude dentinal tubules more effectively in vitro.
