Nav1.8 and Nav1.9 mRNA expression in rat trigeminal ganglion at different interval after molar extraction.
- Author:
Lei ZHANG
1
;
Hong-Chen LIU
;
Dong-Sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; NAV1.8 Voltage-Gated Sodium Channel; NAV1.9 Voltage-Gated Sodium Channel; Neuropeptides; metabolism; Pain, Postoperative; metabolism; RNA, Messenger; genetics; Rats; Rats, Wistar; Sodium Channels; metabolism; Tooth Extraction; Trigeminal Ganglion; metabolism
- From: Chinese Journal of Stomatology 2009;44(5):301-303
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression and function of extraction.
METHODSReal-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1.8, Nav1.9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control.
RESULTSBoth Nav1.8 and Nav1.9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1.8, Nav1.9 mRNA expressions increased by 27% and 24.5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level (P < 0.05), and then the expressions began decreasing from 6 d.
CONCLUSIONSThe pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.
