Effects of Ezrin gene on the proliferation and invasion activity of human salivary gland adenoid cystic carcinoma
10.3760/cma.j.issn.1002-0098.2009.04.003
- VernacularTitle:Ezrin基因在人涎腺腺样囊性癌中的表达及对转移细胞增殖侵袭性的影响
- Author:
You-Yuan WANG
1
;
Wei-Liang CHEN
;
Zhao-Hui YANG
;
Zhi-Quan HUANG
;
Jin-Song LI
;
Chao-Bin PAN
Author Information
1. 中山大学附属第二医院
- Keywords:
Carcinoma,adenoid cystic;
Gene therapy;
Heoplasm metastasis
- From:
Chinese Journal of Stomatology
2009;44(4):203-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of Ezrin in human salivary gland adenoid cystic carcinoma and investigate the effects of Ezrin gene silence on cell proliferation, apoptosis and invasion of adenoid cystic carcinoma (ACC)-M. Methods The expression of Ezrin was detected by immunohistochemistry in normal salivary gland tissue( n = 15 ), pleomorphic adenoma( n = 40) and salivary gland adenoid cystic carcinoma (n = 43). The Ezrin StealthTM RNAi Duplex, containing StealthTM RNAi Negtive Control Duplex were contracted and transfected into ACC-M cells by Lipofectamine TM 2000. The expression levels of Ezrin were detected by RT-PCR and immunohistoehemistry. The cell cycle and apoptosis rate were analyzed by flow cytomtery (FCM). The cell proliferation was detected by methyl thiazolyl tetrazolium(MTT) and cell invasion by Transwell test. Results The positive rate of Ezrin expression in ACC was significantly higher than that in normal salivary gland tissue and plecmorphic adenoma (P <0. 05). After transfection of Ezrin StealthTM RNAi Duplex, the mRNA and protein expression of Ezrin were down-reguLated, the cell proliferation activity was inhibited, the GO-G1 Phase cells were increased, and the apoptosis rate of Ezrin StealthTM RNAi Duplex group was higher than that in control groups and cell invasion ability was decressed. Conclusions Over expression of Ezrin in human salivary gland adenoid cystic carcinoma may promote genesis, development and metastasis of tumors. Ezrin StealthTM RNAi Duplex could efficiently down-regulate the expression of Ezrin gene, and partly inhibite proliferation of ACC-M cells,induce apoptosis and degrease invasion ability of these cells in vitro.
