Celecoxib enhances the lethal effects of bleomycin in human tongue squamous carcinoma cell line Tca8113
10.3760/cma.j.issn.1002-0098.2009.03.005
- VernacularTitle:塞来昔布增强博来霉素对人舌鳞状细胞癌Tca8113杀伤作用的研究
- Author:
Wei-Zhong LI
1
;
Xiao-Yan WANG
;
Zu-Guo LI
;
Jin-Hua ZHANG
;
Yan-Qing DING
Author Information
1. 南方医科大学附属南方医院
- Keywords:
Carcinoma,squamous cell;
Cyclooxygenase 2;
Cyclooxygenase 2 inhibitors
- From:
Chinese Journal of Stomatology
2009;44(3):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of COX-2 inhibitor celecoxib in enhancing the lethal effects of bleomycin in Tca8113 cell line.Methods Tca8113 ceils were treated with different concentrations of celecoxib and bleomycin for 24, 48, 72 h.Methyl thiazolyl tetrazolium assay was used to calculate cell growth inhibition rate and Jin Zheng Jun's method was used to evaluate the interaction of celecoxib and bleomycin on Tca8113 cells.Flow cytometry was used to evaluate the effects of combined use of celecoxib and bleomycin on cell cycle progress and apoptosis.Results Low dose of celecoxib (10 μmol/L, ＜IC50) combined with bleomycin showed synergism or additive lethal effect on Tca8113 cell line.Celecoxib could notably enhance the inhibitory effect of bleomycin on Tca8113 cells by blocking cell cycle progress and thus resulting in the increasing G0/G1 cells [(60.93±0.32) %] distribution and inducing apoptosis [(1.87±0.11) %].Conclusions Low doses of celecoxib could significantly enhance the lethal effect of bleomycin on Tca8113 cells by inhibiting cell growth and proliferation through blocking cell cycle progress and inducing apoptosis.The ways of these interactions on inhibiting Tca8113 cell growth were synergistic or/and additive.
