A preliminary study of murine hemangioma model by delivery of recombinant adeno-associated virus mediated human vascular endothelial growth factor-121 gene
10.3760/cma.j.issn.1002-0098.2009.03.011
- VernacularTitle:重组腺相关病毒携带人血管内皮细胞生长因子121制备小鼠血管瘤模型的初步探讨
- Author:
Zhen-Qi XU
1
;
Yi-Xiang WANG
;
Juan-Hong MENG
;
Wei ZHANG
;
Fu-Yun ZHAO
;
Yu LIU
Author Information
1. 北京大学口腔医学院(口腔医院)
- Keywords:
Hemangioma;
Models,animal;
Vascular endothelial growth factor
- From:
Chinese Journal of Stomatology
2009;44(3):162-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of establishing a marine hemangioma model with injection of recombinant adco-associated virus mediated human vascular endothelial growth factor-121 (rAAV-hVEGF121) gene.Methods rAAV-hVEGF121 was constructed, identified and then implanted to the left back ear of each mouse (1.0×1011VG in 50μl per mouse and 10 nude mice received the injection) , the rights served as controls with an injection of the same volume of phosphate buffered solution(PBS).The skin color and swelling of left back car were observed every other day.Histological examination was carried out after mice were sacrificed 2,4,6,8,12 weeks after injection.Results The rAAV-hVEGF121 was correctly constructed and confirmed by restriction endonuclease analysis, polymerase chain reaction and DNA sequencing analysis.The skin of left back ear became red 2 weeks after injection and gradually exhibited a red lump which was at its utmost 12 weeks after injection.Such phenomena were not observed in right back ear.Histological examinations showed aggregates of endothelial cells by 2 weeks and at 8 weeks the swollen tissue contained many cysts filled with a mass of red cells.CD-34 staining suggested most of the newly-formed cells were endothelial cells.Conclusions A hemangioma model was established in mice with injection of recombinant rAAV-hVEGF121 gene.
