Differential thymosin beta 10 expression levels and actin filament organization in tumor cell lines with different metastatic potential.
- Author:
Cong-rong LIU
1
;
Chun-shu MA
;
Jun-yu NING
;
Jiang-feng YOU
;
Song-lin LIAO
;
Jie ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Actin Cytoskeleton; ultrastructure; Blotting, Northern; Cell Line, Tumor; Humans; Immunohistochemistry; Neoplasm Metastasis; genetics; ultrastructure; RNA, Messenger; analysis; Thymosin; analysis
- From: Chinese Medical Journal 2004;117(2):213-218
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDTo investigate the differential expression levels of thymosin beta 10 (T beta 10) and the corresponding changes of actin filament organization in human tumor cell lines with different metastatic potential.
METHODSFour groups of nine human tumor cell lines with different metastatic potential were analyzed for the amount of T beta 10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin (F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization.
RESULTSIn comparison with non-/weakly metastatic counterparts, T beta 10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines.
CONCLUSIONT beta 10 levels correlate positively with the metastatic capacity in human tumors currently examined. The increasing metastatic potential of tumor cells is accompanied by a loss of F-actin, poorly arranged actin skeleton organizations and presence of F-actin aggregates. There is a consistent correlation between the elevated T beta 10 expression and the disrupted actin skeleton.