Effect of SiO2 on the expression of MMP-9 and TIMP-1 in human alveolar macrophages in vitro.
- Author:
Xiao-bing MA
1
;
Xiu-ling LI
;
Shu-xun SUN
;
Fang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Bronchoalveolar Lavage Fluid; cytology; Cells, Cultured; Humans; Immunohistochemistry; Macrophages, Alveolar; drug effects; metabolism; Male; Matrix Metalloproteinase 9; biosynthesis; Middle Aged; Silicon Dioxide; pharmacology; Silicosis; pathology; Tissue Inhibitor of Metalloproteinase-1; biosynthesis
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):203-205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of SiO(2) on the expression of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) in human alveolar macrophages (AMs) associated with the pathogenesis of silicotic fibrosis.
METHODSAMs were collected from a silicotic patient by bronchoalveolar lavage, and exposed to SiO(2) (50 microg/ml), and cultured in DMEM without serum for different time (2, 6, 12, 18, 24, 36 h). Immunocytochemical method was used to detect the level of expression of MMP-9 and TIMP-1 in AMs.
RESULTSThe expression of MMP-9 in AMs exposed to silica was up-regulated, and reached the peak at 18 h [average optical density: (0.440 +/- 0.021) vs (0.390 +/- 0.011), P < 0.05]. After that, the expression reduced markedly. However, the expression of TIMP-1 of AMs were not significantly different from the control group [average optical density: (0.175 +/- 0.019) vs (0.162 +/- 0.044), P > 0.05].
CONCLUSIONSiO(2) could induce up-expression of MMP-9 in AMs. Degradation of basement membrane by MMP-9 produced by AMs at early stage of lung injury may associate with the immigration of various cells including lung fibroblasts into the injured region.
