Study on inhibitory effect of lycium pigment on lipopolysaccharide-induced uveitis in rats and its mechanism.
- Author:
Jin ZHANG
1
;
Fei-Fei ZHAO
;
Shuang BAI
;
Jie ZHENG
;
Ping ZHENG
;
Gui-Dong DAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Glutathione Peroxidase; genetics; metabolism; Humans; Lipopolysaccharides; adverse effects; Lycium; chemistry; Male; Malondialdehyde; metabolism; Nitric Oxide Synthase Type II; genetics; metabolism; Pigments, Biological; administration & dosage; Plant Extracts; administration & dosage; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; genetics; metabolism; Uveitis; chemically induced; genetics; metabolism; prevention & control
- From: China Journal of Chinese Materia Medica 2013;38(11):1778-1782
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of lycium pigment on lipopolysaccharide (LPS)-induced uveitis in rats and its mechanism.
METHODThe rat uveitis model was established by 30-day oral administration of lycium pigment (50, 100, 200 mg x kg(-1)) and footpad injection of LPS. Ocular tissues were collected for a histopathological inspection. The protein, nitric oxide and ADMA in aqueous humor, level of inducible nitric oxide synthase (iNOS) in retina, activities of serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and content of malondialdehyde (MDA) were determined by using Western blot, ELISA and biochemical methods.
RESULTAccording to the pathological study, lycium pigment (50, 100, 200 mg x kg(-1)) could notably reduce the inflammatory cell infiltration around corpus ciliare matrix of uveitis rats, and the concentration of protein and nitric oxide, and increased ADMA in aqueous humor. Lycium pigment (100, 200 mg x kg(-1)) could significantly inhibit the expression of iNOS in ocular tissues. In addition, lycium pigment (100, 200 mg x kg(-1)) also decrease the activities of serum T-AOC, SOD, GSH-PX, and the content of lipid peroxide MDA.
CONCLUSIONLycium pigment has the inhibitory effect on LPS-induced uveitis in rats. Its mechanism is related to the regulation of nitric oxide/ADMA pathway and the improvement of oxidation resistance.
