Comparison between icariin and genistein in osteogenic activity of marrow stromal cells.
- Author:
Jian ZHOU
1
;
Ke-Ming CHEN
;
Bao-Feng GE
;
Xiao-Ni MA
;
Xiao-Yu GUO
;
Kui CHENG
;
Yu-Hai GAO
;
Li-Juan YAN
;
Wen-Gui SHI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; genetics; metabolism; Animals; Bone Morphogenetic Protein 2; genetics; metabolism; Cell Differentiation; drug effects; Cells, Cultured; Flavonoids; pharmacology; Gene Expression; drug effects; Genistein; pharmacology; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Osteogenesis; drug effects; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(11):1783-1788
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC).
METHODRat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h.
RESULTGenistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) could increase the activity of ALP and the content of Ca, regulate OXS, BMP-2, Runx-2 and Collagen-I mRNA expression.
CONCLUSIONIcariin showed a stronger effect in improving the osteogenic differentiation of rat bone marrow stromal cells than genistein.
