Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus.
- Author:
Xiao-Ping ZHAO
1
;
Zhi-Wei GE
;
Yu-Feng ZHANG
;
Xiao-Hong LAN
;
Bo-Li ZHANG
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. zhaoxiaoping@zjtcm.net
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Drug-Related Side Effects and Adverse Reactions;
Drugs, Chinese Herbal;
chemistry;
toxicity;
Fruit;
chemistry;
Hep G2 Cells;
Humans;
Liver;
drug effects;
Melia;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(11):1820-1822
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
