Preparation of ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong by preparative HPLC.
- Author:
Yao-Kun XIONG
1
;
Shuang LIANG
;
Yan-Long HONG
;
Xiu-Juan YANG
;
Lan SHEN
;
Yan DU
;
Yi FENG
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
- Publication Type:Journal Article
- MeSH:
Benzofurans;
chemistry;
isolation & purification;
Chromatography, High Pressure Liquid;
methods;
Coumaric Acids;
chemistry;
isolation & purification;
Ligusticum;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(12):1947-1950
- CountryChina
- Language:Chinese
-
Abstract:
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (50:50)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.
