Effect of pulchinenoside in regulating FLS SFRP2 expression of RA model rats.
- Author:
Cheng-Gui MIAO
1
;
Jian-Ting YANG
;
Hua-Qi HE
;
Shi-Tang MA
;
Guo-Liang ZHOU
;
Min GAO
;
Jian LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arthritis, Rheumatoid; drug therapy; metabolism; Disease Models, Animal; Gene Expression Regulation; drug effects; Male; Membrane Proteins; genetics; Rats; Rats, Sprague-Dawley; Saponins; pharmacology; Synovial Membrane; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(12):1977-1981
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of pulchinenoside (PULC) in modulating SFRP2 expression in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) model rats.
METHODThe effect of PULC in treating RA rats was evaluated by rat arthritis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene beta-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting.
RESULTPULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regulated, whereas beta-catenin and C-myc gene expressions were significantly down-regulated.
CONCLUSIONPULC can inhibit abnormal proliferation of synovial membrane by modulating Wnt pathway of RA rats.
