Detection of tobacco mosaic virus (TMV) in Rehmannia glutinosa f. hueichingensis by IC-RT-PCR.
- Author:
Lin DU
1
;
Jin-Le XIANG
;
Jin-Ling FAN
;
Xin LI
;
Lei LUO
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Rehmannia; virology; Reverse Transcriptase Polymerase Chain Reaction; methods; Tobacco Mosaic Virus; genetics; immunology; isolation & purification
- From: China Journal of Chinese Materia Medica 2013;38(13):2092-2095
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rapid, sensitive and efficient detection method for tobacco mosaic virus (TMV), and provide technical support of TMV detection of Rehmannia glutinosa f. hueichingensis. The virus-free plantlets could be produced on a large scale to ameliorate breed degeneration caused by viral disease.
METHODSpecific primers were designed based on the conserved region of coat protein(CP) gene of TMV. Immunocapture RT-PCR (IC-RT-PCR) was employed to detect TMV and the sequence of the products was detected.
RESULTThe expected nucleotide acid fragments were amplified by IC-RT-PCR. The homology of nucleotide acid sequence and amino acid sequence were 95.29% and 96.7% between the PCR products and the CP gene of TMV (accession number AY555269).
CONCLUSIONThe method was established for the detection of TMV in R. glutinosa f. hueichingensis by IC-RT-PCR. This detection combined molecular biology technology with immunology, was convenient for a quick, sensitive and simple detection of TMV.
