Preparation and in vitro evaluation of curcumin CTPP-PEG-PCL micelles.
- Author:
Li-Qiao ZHANG
1
;
Li-Biao LUAN
;
Yan WU
;
Dong-Mei WANG
Author Information
1. Department of Pharmaceutics of China Pharmaceutical University, Nanjing 211198, China. zhangliqiao1989@126.com
- Publication Type:Journal Article
- MeSH:
Cell Line;
Cell Proliferation;
drug effects;
Curcumin;
administration & dosage;
chemistry;
pharmacology;
Hepatic Stellate Cells;
drug effects;
Micelles;
Polyesters;
administration & dosage;
chemistry;
Polyethylene Glycols;
administration & dosage;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(13):2109-2113
- CountryChina
- Language:Chinese
-
Abstract:
To synthetize 3-carboxypropyl-triphenylphosponium bromide-polycaprolacton-CTPP-PEG-PC, and prepare curcumin CTPP-PEG-PCL micelles by using the self-assembled emulsion solvent evaporation method, in order to determine the critical micelle concentration (CMC) with the pyrene fluorescent probe technology, detect the particle size, entrapment efficiency (%), morpheme and in vitro release rate, and evaluate the cytotoxicity of hepatic stellate cells with MTT assay. The structure of CTPP-PEG-PCL had been identified by 1H-NMR spectra. Specifically, the CMC of polymer was 2.25 mg x L(-1), the average size was 190 nm, the drug content was (0.66 +/- 0.008) g x L(-1), and the entrapment efficiency was (94 +/- 0.6)%. The in vitro release results showed curcumin micelles had a significant higher inhibition ratio in the growth of hepatic stellate cells than crude curcumin (P < 0.05). This suggested that CTPP-PEG-PCL micelles feature low CMC, high encapsulation efficiency and notable inhibition effect in growth of hepatic stellate cells.
