Study on directional separation of picroside II from extract of traditional Chinese medicine by molecularly imprinted technology.
- Author:
Li-Na YI
1
;
Ke-Qin LI
;
Qiu-Juan WANG
;
Qing-Shan LIU
;
Qing-Long GUO
Author Information
1. China Research Center for Traditional Minority Medicine, Minzu University of China, Beijing 100081, China. yilina0123@163.com
- Publication Type:Journal Article
- MeSH:
Cinnamates;
isolation & purification;
Drugs, Chinese Herbal;
analysis;
Iridoid Glucosides;
isolation & purification;
Medicine, Chinese Traditional;
Molecular Imprinting;
methods
- From:
China Journal of Chinese Materia Medica
2013;38(13):2136-2139
- CountryChina
- Language:Chinese
-
Abstract:
Picroside II, separated from Chinese herbal medicine, is an active compound with neroprotective activity. Molecularly imprinted polymers (MIPs) have high affinity toward template molecules synthesized by molecularly imprinted technology for its specific combined sites, which can overcome the shortcomings of traditional separation methods, such as complex operation and low efficiency. In this paper, MIPs were prepared by precipitation polymerization with picroside II as the template molecule, 1-vinylimidazole (1-Vinyl) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross-linker. The morphology of MIPs was characterized by scanning electronmicroscope (SEM) and its static adsorption capacity was measured by the scatchard equation. The results showed that picroside II MIPs have spherical shape, and most of them are uniform in size. Furthermore, the maximum binding capacity (Q(max)) of MIPs is 3.02 mg x g(-1), higher than that of non-imprinted polymers (NIPs). This result indicated that picroside II MIPs with good morphology and high targeted affinity toward the template molecules can be prepared by precipitation polymerization, which can be used to separate picroside II and its analogies from extract of Chinese herbal medicine. In addition, this method has the advantages of good environment and simple operation, which might offer a novel method for the efficient separation of picroside II in the traditional herbal medicines.
