Apoptosis effects of drug sensitivity leukemia cells induced by nano-realgar.
- Author:
Yong-Sheng WANG
1
;
Si-Tong ZHOU
;
Hu-Lai WEI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Arsenicals; pharmacology; Cell Proliferation; drug effects; Humans; K562 Cells; Leukemia; drug therapy; pathology; Nanotechnology; Proto-Oncogene Proteins c-bcl-2; analysis; Sulfides; pharmacology; Tumor Suppressor Protein p53; analysis
- From: China Journal of Chinese Materia Medica 2013;38(13):2202-2205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore apoptosis-inducing effects of realgar nanoparticle (nano-realgar) on drug-sensitive leukemia cells.
METHODPreparation of nano-realgar was mechanical milled using a high-energy planetary ball mill. Using drug-sensitive leukemia cells (K562) as target cells, MTT assay was used to detect the proliferating activity of K562 cells, and the cellular apoptosis was investigated with double staining of FITC-Annexin V and propidium iodide (PI) by flow cytometry. Flow cytometry (FCM) was employed to detect expression of intracellular Bax, Bcl-2, P-53 protein and the activity of Caspase-3.
RESULTThe raw realgar was made to ultra-fine powder by ball milling, and the average diameter of the nanoparticle was (72.72 +/- 22.18) nm measured with electron microscopes. Nano-realgar significantly inhibited the proliferation of K562 cells, Treated for 24, 48 and 72 hours, the 50% inhibitory concentration (IC50) was 43.48, 20.52, 16.07 mg x L(-1). After exposure to 20 mg x L(-1) and 50 mg x L(-1) nano-realgar for 48 hours, the apoptosis of K562 cells detected by Annexin V/PI staining was increased, the apoptotic rate of K562 cells was 10. 52% and 73.25%. After the target cells were treated with 20 mg x L(-1) and 50 mg x L(-1) nano-realgar for 48 h, the expression of P-53, Bax, Bcl-2 markedly increased in a time and dose-dependent manner. After administration of 20 mg x L(-1) and 50 mg x L(-1) nano-realgar for 48 h, the percentage of BCRP+, P-gp+ and co-expressing P-gp and BCRP cell population in K562 cells incrased dramatically.
CONCLUSIONNano-Realgar significantly induced apoptosis of drug-sensitive leukemia cells.