Effect of neferine on hepatic stellate cells in collagen-I, TIMP-1 and MMP-2.
- Author:
Xian-Mei LI
1
;
Lei DONG
;
Hai-Tao SHI
;
Tian-Jiao GAO
;
Miao JIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzylisoquinolines; pharmacology; Cells, Cultured; Collagen Type I; analysis; genetics; Drugs, Chinese Herbal; pharmacology; Hepatic Stellate Cells; chemistry; drug effects; Matrix Metalloproteinase 2; analysis; genetics; Rats; Tissue Inhibitor of Metalloproteinase-1; analysis; genetics
- From: China Journal of Chinese Materia Medica 2013;38(13):2206-2209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of neferine on Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells.
METHODThe hepatic stellate cell line HSC-T6 was cultured in vitro, and then randomly divided into 5 groups: the control group, the platelet-derived growth factor (PDGF) group and PDGF + neferine (2, 6, 10 micromol x L(-1)) groups. All of the groups were cultured for 48 h, and their cells were collected to extract mRNA and detect Collagen-I, TIMP-1 and MMP-2 expressions with RT-PCR. Their cell supernatants were also collected to determine the protein content of three factors with ELISA.
RESULTCompared with the control group, PDGF could remarkably increase the Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells. Compared with the PDGF group, PDGF + neferine (6, 10 micromol x L(-1)) groups showed a notable decrease in the Collagen-I and mRNA expression and protein secretion along with the increase in the concentration, whereas the PDGF + neferine (2 micromol x L(-1)) group showed no significant change in the Collagen-I and mRNA expression and protein secretion. Compared with the PDGF group, three PDGF + neferine groups showed no notable change in MMP-2 expression and protein secretion.
CONCLUSIONNeferine can inhibit the Collagen-I, TIMP-1 and mRNA protein expression and protein secretion of PDGF-induced HSCs along with the increase in the concentration, but with not remarkable effect on the MMP-2 expression and secretion.