Cloning and bioinformation analysis of flavone synthase II gene of Erigeron breviscapus.
- Author:
Guang-Hui ZHANG
1
;
Cui-Ting LI
;
Jian-Jun WANG
;
Wei ZHANG
;
Jun-Wen CHEN
;
Jian-Wen YANG
;
Sheng-Chao YANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Cloning, Molecular; methods; Computational Biology; methods; Cytochrome P-450 Enzyme System; genetics; metabolism; Erigeron; enzymology; genetics; Genes, Plant; Molecular Sequence Data; Plants, Medicinal; enzymology; genetics; Sequence Alignment
- From: China Journal of Chinese Materia Medica 2013;38(14):2231-2236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEErigeron breviscapus is a medicinal plant with the most developmental potential in Yunnan province, which is belongs to Erigeron genus of Compositae family. Scutellarin, the main active component of Erigeron breviscapus is one of flavone 7-O-glucuronide derivatives, its biosynthesis pathway is still not clear.
METHODFull length cDNA encoding flavone syhthase II gene in E. breviscapus was cloned in this study using R-PCR, 3'-RACE and 5'-RACE.
RESULTThe opening reading frame of FS II cDNA of E. breviscapus is 1 557 bp long and encoding 518 amino acids, designed as EbFS II, which is highly homologous with FS II of Compositae species, like Callistephus chinensis, Cynara cardunculus var. scolymus, Gerbera hybrida, Dahlia pinnata and Lobelia erinus.
CONCLUSIONPhylogenetic analysis showed that EbFS II might has the function of directly converting flavanone to flavone.
