Genetic diversity and group consistency of breeding strains of Erigeron breviscapus determined by AFLP marker.
- Author:
Wei ZHANG
1
;
Jian-Jun WANG
;
Xiang WEI
;
Guang-Hui ZHANG
;
Jian-Wen YANG
;
Dao-Cong WU
;
Sheng-Chao YANG
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; Breeding; Erigeron; genetics; metabolism; Genetic Markers; Genetic Variation; Plants, Medicinal; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(14):2245-2249
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the genetic diversity and breeding strains of the E. breviscapus germplasms, in order to provide theoretical information for Erigeron breviscapus breeding.
METHODThe genetic diversity and genetic structure were assayed to six germplasm resource of E. breviscapus which collected from Yunnna with 11 pairs primers and AFLP molecular marker.
RESULTSix hundred and four amplification bands among 636 DNA bands were from six accession of E. breviscapus, which are about 82.40% of total bands. The six germplasms could be divided into three group at the 0. 706 similarity coefficient level. The first category include QS-1, QS-2 and Dali, Shilin, Kunming population. The second category included wild population of Qiubei. The third category included several sample from different district. The mean genetic similarity coefficient of QS-1 and QS-2 was bigger, genetic similarity coefficient range was smaller, hereditary character was more stable. Molecular system clustering analysis showed that the geographical origin of the same part had relative polymerization phenomenon and its genetic relationship was close. Qiubei was a single group possibly relating to the specific genetic basis.
CONCLUSIONThe analysis of genetic diversity of E. breviscapus by AFLP marker is reliable. The systematic E. breviscapus breeding is feasible.