Open abdomen management treatment of liver injury in rats with abdominal compartment syndrome and sepsis.
- Author:
Jun CHEN
1
;
Jian-an REN
;
Wei-wei ZHANG
;
Guo-sheng GU
;
Chao-gang FAN
;
Xin-bo WANG
;
Jie-shou LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Interleukin-6; metabolism; Intra-Abdominal Hypertension; metabolism; pathology; surgery; Laparotomy; Liver; metabolism; pathology; physiopathology; Rats; Rats, Sprague-Dawley; STAT3 Transcription Factor; metabolism; Sepsis; metabolism; pathology; surgery; Suppressor of Cytokine Signaling Proteins; metabolism; Toll-Like Receptor 4; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Surgery 2011;49(4):335-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the open and closed management treatment of liver injury in rats with sepsis and abdominal compartment syndrome (ACS).
METHODSThe sepsis and ACS rats (n = 72) were randomized divided into two groups. One group used closed management (n = 36), the other accepted the open abdomen management (n = 36). The rats were killed at 1, 6 h, 1, 3, 5, 7 d after operation. Blood was collected for liver function tests. Liver sections assessed pathologically and the expressions of Toll-like receptor 4 (TLR4), tumor necrosis factor (TNF)-α, interleukin (IL)-6, signal transducers actuators of transcription (STAT3) and suppressor of cytokine signaling 3 (SOCS3) of rat livers were examined by RT-PCR.
RESULTSThe early stage after operation, TNF-α and IL-6 concentrations, STAT3 expressions in rat liver were higher in open abdomen rats than the closed management ones (P < 0.05). TLR4 and SOCS3 expressions were lower in open abdomen rats than the closed management ones (P < 0.05). Aspartate aminotransferase, alanine aminotransferase levels also was lower in open abdomen ones (P < 0.05).
CONCLUSIONSThe randomized study demonstrates that open abdomen management could improve liver regeneration in the early stage after operation. Also open abdomen could reduce inflammatory response by reducing TLR4 expressions.
