Expressions of type I and type III collagens and tenascin-C in rat bone mesenchymal stem cells co-cultured with ligament fibroblasts.
- Author:
Lei ZHANG
1
;
Huaiqing CHEN
;
Wang XIONG
;
Tran NGUYEN
Author Information
1. Institute of Biomedical Engineering, West China Center of Medical Scicences, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cells, Cultured;
Coculture Techniques;
Collagen Type I;
metabolism;
Collagen Type III;
metabolism;
Fibroblasts;
cytology;
Ligaments, Articular;
cytology;
Mesenchymal Stromal Cells;
cytology;
RNA, Messenger;
metabolism;
Rats;
Tenascin;
metabolism
- From:
Journal of Biomedical Engineering
2007;24(4):846-851
- CountryChina
- Language:Chinese
-
Abstract:
Bone marrow mesenchymal stem cells (BMSCs) can be directed to differentiate into a variety of cell types depending on their micro-environment. In this study, rat BMSCs were co-cultured with rat ligament fibroblasts during different time courses. The mRNA expressions of type I, type III collagens and tenascin-C were measured by real time RT-PCR, and the corresponding protein levels of type I and type III collagens by radioimmunoassay. Results show that the mRNA expressions of type I and type III collagens in the BMSCs were 2 times up-regulated after a 6-day co-culture, and the relative mRNA expressions of type I and type III collagens were 3.9 +/- 0.2 and 1.9 +/- 0.2, while they were 1.9 +/- 0.3 and 0.8 +/- 0.1 in the control groups, respectively. The protein syntheses of these two collagens were also increased after a 12-day co-culture; the type I and type III collagens synthesis were 13.6 +/- 1.3 ng/microg and 5.9 +/- 0.5 ng/microg in co-culture groups and 12.4 +/- 0.8 ng/microg and 5.0 +/- 0.4 ng/microg in their control groups, respectively. Likewise, there was a 2 times enhancement in tenascin-C mRNA expression after the 12-day co-culture (0.07 +/- 0.02 by control group and 0.14 +/- 0.02 by co-culture group, P < 0. 05). These data suggest that the presence of the ligament fibroblast promotes the syntheses of type I and the III collagens and tenascin-C in the rat BMSC.
