The drug-resistant mechanism of clinical non-fermenting bacilli producing IMP-1 metalloenzyme.
- Author:
Xi CHENG
1
;
Wenxiang JIA
;
Baozhong DU
;
Wei ZENG
;
Mei KANG
;
Zairong ZHANG
;
Yi XIE
;
Weiqing YANG
;
Hong FAN
;
Chuanmin TAO
;
Lei ZHANG
Author Information
1. Department of Microbiology, West China School of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Acinetobacter baumannii;
drug effects;
genetics;
Anti-Bacterial Agents;
pharmacology;
Ceftazidime;
pharmacology;
Drug Resistance, Multiple, Bacterial;
genetics;
Fermentation;
Imipenem;
pharmacology;
Mercaptoethanol;
pharmacology;
Microbial Sensitivity Tests;
Pseudomonas aeruginosa;
drug effects;
genetics;
beta-Lactamases;
genetics
- From:
Journal of Biomedical Engineering
2007;24(4):876-879
- CountryChina
- Language:Chinese
-
Abstract:
A total of 50 clinical imipenem-resistant isolates of Pseudomonas aeruginosa and Acinetobacter baumannii were subjected to the ceftazidime-2- mercaptoethanol -double-disk synergy test and to the PCR assays with primers specific for bla(IMP-1). After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii. Only one Pseudomonas aeruginosa was found to carry bla(IMP-1), and bla(Int1) at the same time. This is the first ascertainment of IMP-1 producing Pseudomonas aeruginosa isolate carrying bla(IntI1) in West China, which is of significance to the research on the clinical spread of these drug-resisitant genes.
