Effects of laminar shear stress on the expression of IL-8 receptor CXCR1 in endothelial cells.
- Author:
Longjuan XUE
1
;
Chang YU
;
Xiaoheng LIU
;
Yi LAI
;
Ye ZENG
;
Yi ZHANG
Author Information
1. Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Endothelial Cells;
cytology;
metabolism;
Humans;
RNA, Messenger;
biosynthesis;
genetics;
Receptors, Interleukin-8A;
genetics;
metabolism;
Shear Strength;
Stress, Mechanical;
Umbilical Veins;
cytology
- From:
Journal of Biomedical Engineering
2007;24(5):1054-1057
- CountryChina
- Language:Chinese
-
Abstract:
This study was conducted to elucidate the effects of different fluid shear stress on the expression of IL-8 receptors CXCR1 in endothelial cells. The HUVEC cell lines, EA. Hy926 cells, were cultured in vitro, exposed to 5.56, 10.02, and 15.27 dyn/cm2 laminar shear stress, respectively, and then the shear stress grous were each investigated at the time-points of 0h, 1h, 2h, 4h and 8h. Western blotting was used for detecting the expression of IL-8 receptor CXCR1 at the time-points. The results showed that, under 5.56 dyn/cm2 shear stress, the expression of CXCR1 increased with time significantly (P < 0.01). The maximum expression of CXCR1 appeared at 4 h and was 2.2 times that of the control. When exposed to 10.02 dyn/cm2, the expression of CXCR1 increased gradually with time and finally remained at a constant higher level. When exposed to 15.27 dyn/cm2, CXCR1 expression decreased significantly with time (P < 0.01). The minimum CXCR1 expression appeared at 8 h and was 62. 59 percent of that of the control. These results indicate that the expression of CXCR1 in endothelial cell is regulated by laminar shear stress.
