Effect of mechanical stimulation on the invasion in salivary adenoid cystic carcinoma.
- Author:
Haibo HE
1
;
Xiufa TANG
;
Longjiang LI
;
Liang LI
;
Chengge HUA
;
Dengqi HE
Author Information
1. Department of Stomatology, Subei People's Hospital of Jiangsu Provine, Yangzhou 225001, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Adenoid Cystic;
metabolism;
pathology;
Cell Line, Tumor;
Humans;
Image Processing, Computer-Assisted;
Matrix Metalloproteinase 9;
metabolism;
Microscopy, Confocal;
Neoplasm Invasiveness;
Physical Stimulation;
Salivary Gland Neoplasms;
metabolism;
pathology;
Stress, Mechanical
- From:
Journal of Biomedical Engineering
2007;24(5):1065-1088
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to assess the effects of cyclic biaxial mechanical stimulation on the invasion capacity in vitro and on the expression of MMP-9 in the salivary adenoid cystic carcinoma (SACC) high metastatic cell lines ACC-M, and in the SACC low metastatic cell lines ACC-2. We observed the functions of mechanical stimulation in the invasion of cell in vitro and investigated the mechanism involved in the invasion of SACC. Mechanical stimuli were applied to the cells for periods of 1, 3 and 6 hours daily, lasting 2 days. The mechanical stimuli applied to the cells were 4000 micro strain and 1000 micro strain, at a frequency of three hertz. Unstrained cells were used as control. The expressions of MMP-9 on ACC-M and ACC-2 were studied with laser scanning confocal microscope and image analysis software. In vitro invasion capacity of ACC-2 and ACC-M cells was determined by Transwell. The results showed the expression of MMP-9 on the cell of ACC-M was obvious by higher than that on ACC-2. MMP-9 was closely related with the invasion and metastasis of SACC. The mechanical stimulation changed the expression of MMP-9 and the invasion capacity in different duration of mechanical stimulations. Mechanical stimulation can adjust the invasion capacity and expression of MMP-9. It can promote metastasis of the cancer. Moreover, some protein other than MMP-9 may play an important role in the metastasis of the cancer.
