Studies on the extraction of DNA using carboxyl-coated magnetic particles and quantification using fluorescent dye.
- Author:
Yongjie MA
1
;
Hongchen GU
;
Weiming ZHENG
Author Information
1. National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Shanghai Jiaotong University, Shanghai 200030, China.
- Publication Type:Journal Article
- MeSH:
Carbon Dioxide;
chemistry;
DNA;
blood;
isolation & purification;
Ferric Compounds;
chemistry;
Fluorescent Dyes;
Humans;
Magnetics;
Nanoparticles;
Organic Chemicals;
Particle Size;
Staining and Labeling
- From:
Journal of Biomedical Engineering
2007;24(5):1093-1096
- CountryChina
- Language:Chinese
-
Abstract:
Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I, the quantification of double-strand DNA exhibits the advantages of high sensitivity,good repeatability and convenience.
