Study on a novel vascular stent material (titanium oxide, Ti-O) coated with albumin and heparin: is it hemocompatible with fibrinogen.
- Author:
Jie SONG
1
;
Xi WU
;
Nan HUANG
;
Biao XU
;
Fengjuan JING
;
Junying CHEN
;
Yongxiang LENG
;
Ping YANG
;
Jin WANG
;
Hong SUN
;
Ansha ZHAO
Author Information
1. Department of Cardiology, Nanjing Drum Tower Hospital Affiliated to School of Medicine Nanjing University, Nanjing 210008, China.
- Publication Type:Journal Article
- MeSH:
Albumins;
chemistry;
Animals;
Coated Materials, Biocompatible;
pharmacology;
Dogs;
Fibrinogen;
chemistry;
Heparin;
chemistry;
Materials Testing;
methods;
Prosthesis Design;
Stents;
Surface Properties;
Titanium;
chemistry
- From:
Journal of Biomedical Engineering
2007;24(5):1097-1101
- CountryChina
- Language:Chinese
-
Abstract:
The functional hemocompatibility between fibrinogen (FIG) and a novel vascular stent material (Ti-O film fixed with albumin and heparin) was investigated as follows: (1) Preparing the new biologic material (Ti-O) film; (2) Coating albumin and heparin on the Ti-O film; (3) Testing platelets (PL) adsorption; (4) Determining FIG adhesion number by use of enzyme linked immunoassay (ELISA); (5) Implanting the films from the test group of Ti-O film and from the comparison group of stainless steel (SS) film into the left and right femoral arteries respectively in 4 dogs. It was proved that albumin and heparin were fixed on Ti-O film. After 6 months, the femoral arteries of the dogs were resected. In the test group of Ti-O film coated with albumin and heparin, few PL adhered to the coat, their form did not change, and no thrombus was found by scanning electron microscopy; the result was better than that of plain Ti-O film, and was much better than that of SS film. Ti-O maintained normal transformation condition of FIG, and no C terminal of gamma chain in FIG was revealed. As it is known whether the hemocompatibility of a biomaterial is good depends upon its adsorption of FIG, and Ti-O has excellent reaction on albumin and heparin by chemical processes. In this study, the Ti-O film coated with albumin and heparin further reduced the absorption of FIG and PL when compared against the plain Ti-O film. So the Ti-O film coated with albumin and heparin has the insistent and permanent anticoagulant character.
