Use of nanoparticles to monitor human mesenchymal stem cells transplanted into penile cavernosum of rats with erectile dysfunction.
10.4111/kju.2015.56.4.280
- Author:
Jae Heon KIM
1
;
Hong Jun LEE
;
Seung Hwan DOO
;
Won Jae YANG
;
Dongho CHOI
;
Jung Hoon KIM
;
Jong Ho WON
;
Yun Seob SONG
Author Information
1. Department of Urology, Soonchunhyang University Seoul Hospital, Soonchunhyang University College of Medicine, Seoul, Korea. yssong@schmc.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Erectile dysfunction;
Magnetic resonance imaging;
Mesenchymal stem cell transplantation;
Nanoparticles
- MeSH:
Animals;
Contrast Media/pharmacology;
Dextrans/*pharmacology;
Disease Models, Animal;
Drug Delivery Systems/methods;
*Erectile Dysfunction/diagnosis/etiology/therapy;
Magnetic Resonance Imaging/methods;
*Magnetite Nanoparticles;
Male;
Mesenchymal Stem Cell Transplantation/*methods;
Monitoring, Physiologic/methods;
Penis/*innervation;
*Peripheral Nerve Injuries/complications/diagnosis/physiopathology/therapy;
Rats;
Suspensions;
Treatment Outcome
- From:Korean Journal of Urology
2015;56(4):280-287
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was performed to examine the treatment of erectile dysfunction by use of superparamagnetic iron oxide nanoparticles-labeled human mesenchymal stem cells (SPION-MSCs) transplanted into the cavernous nerve injured cavernosa of rats as monitored by molecular magnetic resonance imaging (MRI). MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were divided into three groups of 10 rats each: group 1, sham operation; group 2, cavernous nerve injury; group 3, SPION-MSC treatment after cavernous nerve injury. Immediately after the cavernous nerve injury in group 3, SPION-MSCs were injected into the cavernous nerve injured cavernosa. Serial T2-weighted MRI was done immediately after injection and at 2 and 4 weeks. Erectile response was assessed by cavernous nerve stimulation at 2 and 4 weeks. RESULTS: Prussian blue staining of SPION-MSCs revealed abundant uptake of SPION in the cytoplasm. After injection of 1x10(6) SPION-MSCs into the cavernosa of rats, T2-weighted MRI showed a clear hypointense signal induced by the injection. The presence of SPION in the corpora cavernosa was confirmed with Prussian blue staining. At 2 and 4 weeks, rats with cavernous nerve injury had significantly lower erectile function than did rats without cavernous nerve injury (p<0.05). The group transplanted with SPION-MSCs showed higher erectile function than did the group without SPION-MSCs (p<0.05). The presence of SPION-MSCs for up to 4 weeks was confirmed by MRI imaging and Prussian blue staining in the corpus cavernosa. CONCLUSIONS: Transplanted SPION-MSCs existed for up to 4 weeks in the cavernous nerve injured cavernosa of rats. Erectile dysfunction recovered and could be monitored by MRI.
