Efficient packaging retrovirus and construction of transgenic chicken technical platform.
- Author:
Chaolai MAN
1
;
Qing ZHANG
;
Yan CHEN
;
Dahai ZHU
Author Information
1. Department of Biotechnology, Harbin Institute of Technology, Harbin 150001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Genetically Modified;
Chick Embryo;
Chickens;
genetics;
DNA Primers;
Genetic Vectors;
genetics;
Retroviridae;
genetics;
Vesicular stomatitis Indiana virus;
genetics
- From:
Journal of Biomedical Engineering
2007;24(5):1111-1117
- CountryChina
- Language:Chinese
-
Abstract:
Transgenic chicken and oviduct bioreactor are growing to be one of the hotspot of scientific study in the field of biology. The most successful method of producing transgenic chicken is pseudotyped retrovirus vector system, but no one has reported the production of transgenic chicken by retrovirus system recently in our country. In order to accelerate our study in this field, we introduced the relevant technical methods such as packaging retrovirus and vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retrovirus, optimizing the conditions of packaging retrovirus, concentrating VSV-G pseudotyped retrovirus, helper virus assays, and microinjection of retrovirus. Furthermore, we successfully conducted in vivo study for detecting the marker gene EGFP of chicken embryo as well as in vitro study for detecting that gene of chicken embryo myoblast (CFM), thus we have provided an applied technical platform for studies of transgenic chicken in the future.
