Effect of EBV immediate-early protein Zta on the cell cycle of Daudi cells and its mechanisms.
- Author:
Qing-wei GUO
1
;
Jin-dong GUO
;
Xue-mei LIU
;
Yun-ze LANG
;
Hong-xia ZHANG
;
Guo-sheng JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; genetics; Cell Division; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21; metabolism; E2F1 Transcription Factor; metabolism; Genetic Vectors; Herpesvirus 4, Human; genetics; Humans; Immediate-Early Proteins; genetics; Retinoblastoma Protein; metabolism; Trans-Activators; genetics; Transcriptional Activation; Viral Proteins; genetics
- From: Chinese Journal of Hematology 2012;33(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of EBV immediate-early protein Zta on cell cycle of Daudi cells and the involved mechanisms.
METHODSThe expression vector encoding Zta was constructed and electroporated into Daudi cells. Flow cytometric analysis was used to detect the cell cycle, Western blot to the protein levels of p21, Rb and E2F-1.
RESULTSThe vector was constructed successfully, the expression of Zta protein inhibited the proliferation of Daudi cells and promoted cell cycle from G(0)/G(1) phase \[(30.0 ± 3.4)%\] to S phase \[(47.7 ± 1.1)%\]. Meanwhile, Rb expression was significantly downregulated, E2F-1 and p21 expression upregulated by Zta.
CONCLUSIONZta could promote G(0)/G(1) phase to S phase transition in Daudi cells, which might be associated with the reduced expression of Rb and increased expression of E2F-1 and p21 protein.
