Screen of phosphopeptide specific for acute leukemia.
- Author:
Hong-ling PENG
1
;
Chong-wen DAI
;
Guang-sen ZHANG
;
Ting-lei GU
;
Jian YU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Humans; Immunoprecipitation; Leukemia, Myeloid, Acute; genetics; metabolism; Neoplasm Proteins; analysis; Phosphopeptides; analysis; Phosphorylation; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; metabolism; Proteomics; Tandem Mass Spectrometry
- From: Chinese Journal of Hematology 2012;33(3):163-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen phosphopeptide specific for acute leukemia.
METHODSMononuclear cells from bone marrow were collected from 16 newly diagnosed acute lymphoblastic leukemia (ALL) and 20 acute myeloid leukemia (AML) patients. Peptides were extracted and purified, analyzed by immunoprecipitation and liquid chromatography coupled with tandem mass spectrometry (LC-MS).
RESULTS(1) Non-receptor tyrosine kinase family members Fyn, Yes, Src widely expressed in acute leukemia; (2) Some phosphopeptides, including non-receptor tyrosine kinase family members Abl/iso1 and Abl, non-receptor Ser/Thr protein kinase family members Bcr, JNK2, JNK2 iso2, Adaptor/scaffold members Cas-L, Cbl, CrkL CENTD1 (Centaurin delta1) ZO2, transcriptor GFR-1 and phosphatase SHIP-2 were detected in Ph positive ALL, but not in other kinds of ALL. (3) Hck, Lyn and Fgr selectively expressed in AML (except AML-M(3)).
CONCLUSIONSome phosphopeptides were specific for ALL and AML, and may be useful for diagnosis and therapy of acute leukemia.
