A novel diagnostic measure of platelet-specific antibody in immune thrombocytopenia.
- Author:
Xue-li ZHOU
1
;
Shi YAN
;
Qiang LI
;
Peng LI
;
Ze-ping ZHOU
;
Ren-chi YANG
Author Information
- Publication Type:Journal Article
- MeSH: Autoantibodies; analysis; immunology; Enzyme-Linked Immunosorbent Assay; methods; Female; Humans; Male; Platelet Glycoprotein GPIIb-IIIa Complex; immunology; Platelet Glycoprotein GPIb-IX Complex; immunology; Platelet Membrane Glycoproteins; immunology; Sensitivity and Specificity; Thrombocytopenia; diagnosis; immunology
- From: Chinese Journal of Hematology 2012;33(3):200-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the platelet glycoprotein-specific antibodies in serum of thrombocytopenia patients and evaluate its diagnostic value for immune thrombocytopenia.
METHODAnti-GPIIb/IIIa, GPIb/IX and GPIa/IIa antibodies were assayed by ELISA kit (PAKUTO) in patients with thrombocytopenia.
RESULTSThe sensitivity and specificity of PAKAUTO in immune thrombocytopenia were 44.0% and 95.7%, respectively. The values of positive and negative predictions were 98.0% and 26.2%, respectively. Among those PAKAUTO positive patients, positive rates of GPIIb/IIIa, GPIa/IIa and GPIb/IX were 87%, 35% and 10%, respectively. The positive rate of patients not received immune suppressive agents (58.5%) was significantly higher than those received immune suppressive agents (26.9%) (P < 0.01). The positive rate of patients with platelet count ≤ 20 × 10(9)/L (51.6%) was significantly higher than those with platelet count > 20 × 10(9)/L (27.8%) (P < 0.01). The positive rate of patients with secondary immune thrombocytopenia (66.7%) was significantly higher than those with primary immune thrombocytopenia (41.7%) (P < 0.05).
CONCLUSIONThe highly specific method (PAKAUTO) could effectively differentiate immune or non-immune thrombocytopenia and be applied to diagnosis of immune thrombocytopenia.
